Multi-Parametric Live Cell Microscopy of 3D Tissue Models

Current state-of-the-art 3D tissue models and their compatibility with live cell imaging.- Simultaneous Phosphorescence and Fluorescence Lifetime Imaging by Multi-Dimensional TCSPC and Multi-Pulse Excitation.- Quantitative live cell FLIM imaging in three dimensions.- Three-dimensional tissue models and available probes for multi-parametric live cell microscopy: a brief overview.- Fabrication and handling of 3D scaffolds based on polymers and decellularized tissues.- Multi-parametric imaging of hypoxia and cell cycle in intestinal organoid culture.- Imaging of intracellular pH in tumor spheroids using genetically encoded sensor SypHer2.- Application of fluorescence lifetime imaging (FLIM) to measure intracellular environments in a single cell.- Quantitative imaging of Ca2+ by 3D-FLIM in live tissues.- Live cell imaging of viscosity in 3D tumour cell models.- Live imaging of cell invasion using a multicellular spheroid model and lightsheet microscopy.- Raman imaging microscopy for quantitative analysis of biological samples.

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• Biotecnologia
• Biofísica
• Engenharia biomédica
• Análise de espectro, spectrochemistry, espectrometria de massa

3D live cell imaging;3D tissue models;Applications Multi-parametric imaging;Autofluorescence imaging in cancer;Ca2+ imaging neural;Multi-parametric imaging cancer;Multi-parametric imaging of pH;Multi-parametric imaging viscosity cancer;Multi-parametric quantitative imaging;Quantitative imaging intestinal organoid;Biological Microscopy