Flow Cytometry Basics for the Non-Expert

Flow Cytometry Basics for the Non-Expert

Bonnevier, Jody; Goetz, Christine; Hammerbeck, Christopher

Springer International Publishing AG

11/2018

219

Dura

Inglês

9783319980706

15 a 20 dias

530


ebook

Descrição não disponível.
1. Flow Cytometry: Definition, History, and Uses in Biotechnology.- 2. Physics of a Flow Cytometer.- 3. The Language of Flow Cytometry and Experimental Setup.- 4. Fluorochrome Choices for Flow Cytometry.- 5. Compensation.- 6. Primary and Seconardy Antibodies and Their Use in Flow Cytometry.- 7. Experimental Considerations with Data Sets as Examples.- 8. Cell Enrichment.- 9. Surface and Intracellular Staining Protocols for Flow Cytometry.- 10. Troubleshooting.
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fluorochromes;PMT;dot plots;antibodies;flow cytometry